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Title   사람 위암 및 폐암세포주에서 Buthionine Sulfoximine 에 의한 Cispltin 획득내성의 극복 ( Overcome of Acquired Resistance to Cisplatin by Buthionine Sulfoximine in Human Stomach and Lung Cancer Cell Linces )
Publicationinfo   1993 Jan; 025(01): 1-9.
Key_word   Buthionine sulfoximine, Acquired resistance to cisplatin, Human stomach cancer cell line, Human lung cancer cell line
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Abstract   The overcoming effects of buthionine sulfoximine(BSO), a specific. inhibitor of glutathione synthesis. on the acquired resistance to cisplatin were investigated using MTT assay against human stomach adenocarcinoma cell line(MKN-45), human pulmonary adenocarcinoma cell line(PC-14), and their sublines resistant ta cisplatin(MKN/CDDP and PC/CDDP). MKN/CDDP and PC/CDDP were established by stepwise exposure of MKN-45 and PC-14 to cisplatin. MKN/ CDDP and PC/CDDP were 10.9 and 42.9 fold more resistant to cisplatin, respectively, compared to the respective parent cell line in terms of IC. BSO at a concentration of 100 PM did not in- hibit the survivals of MKN-45, PC-14, MKN/CDDP and PC/CDDP. The survivals of MKN/ CDDP and PC/CDDP were significantly inhibited by the addition of BSO compared to cisplatin alone. The survivals of MKN-45 and PC-14 were also inhibited by the combined use of BSO and cisplatin, although the inhibition rates in MKN-45 and PC-l4 were lower than those on MKN/ CDDP and PC/CDDP, respectively. The overcoming effects of BSO-mediated glutathione de- pletion were evaluated in terms of dose modification factor(DMF) DMFs in MKN-45, PC-14, MKN/CDDP and PC/CDDP were 2.59, 1.94, 3.51 and 5.98, respectively. These results demonstrating that acquired resistance to cisplatin was significantly overcome by the addition of BSO suggest that BSO may be a potential clinical chemosensitizer to overcome the acquired resistance to cisplatin.
저 자   홍원선(Weon Seon Hong),김창민(Chang Min Kim),이춘택(Choon Taek Lee),김유철(You Cheoul Kim),임영혁(Young Hyuk Im),이진오(Jhin Oh Lee),강태웅(Taik Koo Yun),홍석일(Seok Il Hong)